Fig. 2. Effect of bacterial PAMPs and allergic mediators on mRNA expression of interleukin (IL)-1β, IL-6 and IL-8 in human PDL fibroblasts. (A) The human PDL fibroblasts were stimulated with 20 µg/ml LPS, 50 µg/ml PGN, 1 µM trypsin, 1 Unit thrombin, 50 µg/ml GCE, or 50 µg/ml HDM for 3 h. The total mRNA of the stimulated human PDL fibroblasts was extracted and amplified with IL-1β, IL-6, IL-8, and GAPDH specific primers. (B~D) The expression of IL-1β, IL-6, and IL-8 was quantified after the value was normalized to expression level of GAPDH. Data are a mean±SE of values from more than five independent experiments. ^*p<0.05, ^**p<0.01 versus control.