Figure 5.

Effect of TPA on ROCK, PKCα, and PKCζ expression and adducin phosphorylation in OHCs. (A) Levels of ROCK expression were not changed, but labeling of nuclear p-cofilin decreased significantly in OHCs treated with TPA. LPA, in contrast, increased p-cofilin labeling in the cytoplasm and nucleus of OHCs, whereas BIM-1 significantly decreased LPA-induced cofilin phosphorylation. (B) Whereas TPA increased expression of both PKCα and PKCζ, BIM-1 inhibited this effect only for PKCα. Similarly, TPA induced adducin phosphorylation at both Ser-726 and Thr-445, but treatment with BIM-1 reduced adducin phosphorylation only at Ser-726. In all images, scale bar = 10 μm.