Figure 6.

Initial chemical states and responses to the growth factors. (A–C) Distributions of the chemical compositions of single cells before and during 2 h of stimulation with HRG (A) or EGF (B). Distributions of cells in the resting condition for 2 h (C) are shown for comparison. Blue and red cells were grouped into Ra (blue) or Rb (red) before stimulation (A and B) or at the first measurement (C). Ellipses show the distributions of the cell states in the corresponding conditions. (D) State transition diagrams between the first and last measurements. The transition rates were estimated from the weighted distributions of single Raman spectra to the two states using the bootstrap method. Numbers indicate the medians of the transition probabilities, with the 90% confidence intervals in parentheses. Because the fraction of cells was small, it was difficult to accurately estimate the confidence intervals for the transition rates from the Ra state in (B). To see this figure in color, go online.