ZipA is required for the anticorrelated coupling of MinCDE and FtsZ. (A) Confocal fluorescence micrographs showing MinCDE-FtsZ waves (labeled proteins: eGFP-MinC and FtsZ-Alexa647) observed upon joint reconstruction on bilayers without ZipA. (B) Fluorescence intensity profiles of MinC and FtsZ acquired from the image shown in (A). In the absence of ZipA, a small fraction of FtsZ was found to be transported with the MinCDE wave by binding to MinC. In contrast to the situation described for ZipA-SLBs, where anticorrelated FtsZ and MinCDE patterns were observed, here FtsZ colocalizes with MinC, matching its profile. To see this figure in color, go online.