Figure 4.

Trace at the starting point of the actual transport. (A) The position-versus-time plots were fitted by exponential functions resulting in the stated decay times (see Fig. 3 legend). The distances of the abrupt movement can be determined to be 119 nm, 64 nm, and 196 nm for x, y, and z directions, respectively, resulting in a total distance of 238 nm. (B) Stiffness parameters resulting from thermal fluctuations. Mean values before and after t = t_rest are indicated by the dashed lines in the corresponding color. (C and D) Mechanistic model for the release of a locking bond, A (C), and the start of transport through a second bond, B (D), at t > t_rest. (E and F) Sketch of a corresponding Kelvin-Voigt model with stiffness parameters κ_A and κ_B, friction parameters γ_A and γ_B, and equilibrium positions r_A and r_B. To see this figure in color, go online.