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Analysis of orientational changes. (A) Mechanistic sketch of bead attached to filopodium illustrating the angle φ. (B) 2D elliptical-position histogram perpendicular to the filopodium axis with minimal and maximal connection stiffness, κmin and κmax. The angle φ indicates the tilt of the fluctuation volume. (C) Change in connection stiffness, κc(ϕ), for different angles of analysis and at three different times during transport. To see this figure in color, go online.
