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Kinetic parameters for WT and L93A Pfk-2
| Pfk-2 | KM (μM) MgATP | KM (μM) F6P | kcat (s−1) |
|---|---|---|---|
| WT (3 μM) | 14 + 4 | 45 ± 5 | 65 ± 5 |
| L93A (3.3 μM) | ND | ND | ∼2 |
| L93A (15 μM) | 20 ± 4 | 34 ± 3 | 12 ± 2 |
| L93A+F6Pa (3.3 μM) | 19 ± 4 | 43 ± 5 | 40 ± 3 |
ND, not determined.
The protein was preincubated with 1 mM F6P for 40 min at 20°C before kinetic measurements.
