Figure 5.

MPP1 knockdown causes a shift in the temperature of phase separation of GPMVs. (A) Representative images and (B) quantification of phase separation in GPMVs isolated from different cell types, measured at various temperatures. Phase separation in vesicles obtained from knockdown cells is observable approximately up to 15°C, whereas control vesicles remain phase separated up to 17°C. Points are an average of ±10 vesicles per cell type, representative of three independent experiments. (C) Average temperature at which phase separation occurs (T_misc, defined as the temperature at which 50% of vesicles remain phase separated) of GPMVs isolated from different cell types. T_misc of vesicles isolated from MPP1-KnD cells is ∼2°C lower compared to control. Differences were considered statistically significant at ^∗∗p < 0.01. To see this figure in color, go online.