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. 2015 Mar 10;25(5):561–573. doi: 10.1038/cr.2015.30

Figure 4.

Figure 4

ERK interacts with FBW7. (A, B) Immunoblot (IB) analysis of whole-cell lysates (WCL) and immunoprecipitates (IP) from 293T cells transfected with Flag-WT-FBW7, or HA-ERK1 or together. Thirty hours post-transfection, cells were pretreated with 15 μM MG132 for 10 h before harvesting. (C, D) Immunoblot analysis of WCL and immunoprecipitates from the indicated SW1990 cells stably expressing the Flag-ERK1 construct. Cells were pretreated with 15 μM MG132 for 10 h before harvesting. (E) SW1990 cell extracts were immunoprecipitated with an antibody against FBW7 or control IgG and analyzed by immunoblot analysis.