Table 1.

PCR primers for amplification of artificial microRNAs

artificial microRNA		primer sequence	product size(bp)
miR-polα	1st round	F: 5-TGCTGTTGACAGTGAGCGCGCAGATCATGTGTGAGCTAAATAGTGAAGCCACAGATGTA-3	97
		R: 5-TCCGAGGCAGTAGGCATGCAGATCATGTGTGAGCTAAATACATCTGTGGCTTCACTATT-3
	2nd round	F: 5-AGATCTGATCCAAGAAGGTATATTGCTGTTGACAGTGAGCG-3	142
		R: 5-GGATCCATCGTAGCCCTTGAAGTCCGAGGCAGTAGGCA-3
miR-polδ	1st round	F: 5-TGCTGTTGACAGTGAGCGACGGGACCAGGGAGAATTAATATAGTGAAGCCACAGATGTA-3	97
		R: 5-TCCGAGGCAGTAGGCAGCGGGACCAGGGAGAATTAATATACATCTGTGGCTTCACTATA-3
	2nd round	F: 5-AGATCTGATCCAAGAAGGTATATTGCTGTTGACAGTGAGCG-3	142
		R: 5-GGATCCATCGTAGCCCTTGAAGTCCGAGGCAGTAGGCA-3
miR-polε	1st round	F: 5-TGCTGTTGACAGTGAGCGAGGTTCCCACTTGCTGCTCAATTAGTGAAGCCACAGATGTA-3	97
		R: 5-TCCGAGGCAGTAGGCACGGTTCCCACTTGCTGCTCAATTACATCTGTGGCTTCACTAAT-3
	2nd round	F: 5-AGATCTGATCCAAGAAGGTATATTGCTGTTGACAGTGAGCG-3	142
		R: 5-GGATCCATCGTAGCCCTTGAAGTCCGAGGCAGTAGGCA-3

The bold italics indicate restriction endonuclease sites for cloning.