Table 3.

Quantitative PCR primers for determination of mRNA expression levels of polymerase$\mathbf{\alpha }$, $\mathbf{\delta }$, $\mathbf{\epsilon }$, AFP and$\mathbf{\beta }$-actin

gene detected	gene code	primer sequence	product size(bp)
polymerase α	NM_016937.3	F: 5- TTCTGACAAGTCCCTGTACACC −3	240
		R: 5- TAGGATTTCACGGCACAACCA −3
polymerase δ	NM_002691.3	F: 5- CCCTACGTGATCATCAGTGC −3	107
		R: 5- AGGTAGTACTGCGTGTCAATGG −3
polymerase ε	NM_006231.2	F: 5- CCTACGACTCCTCTGCCATC −3	219
		R: 5- CGTAGTGCTGGGCAATGTTC −3
AFP	NM_001134.1	F: 5- ACCTCGTCGGAGCTGATGG −3	204
		R: 5- TGGCCTCCTGTTGGCATATG −3
β-actin	NM_001101.3	R: 5- CCGATCCACACGGAGTACTT −3	406