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. 2015 Apr 29;6:6840. doi: 10.1038/ncomms7840

Figure 1. Tumour-secreted factors favour BMDC differentiation towards high Id1-expressing MDSCs but not DCs.

Figure 1

Flow cytometry analysis of splenic populations from B16F10 melanoma-implanted mice (day 21 post implantation). (a) Frequency and absolute numbers of DCs (unpaired t-test **P<0.01). (b) Frequency and absolute numbers of MDSCs (unpaired t-test, ***P<0.001). Flow cytometry analysis of spleens from E0771 mammary adenocarcinoma-implanted mice (day 21 post implantation) for (c) DC absolute numbers and (d) MDSC absolute numbers compared with control mice (unpaired t-test, **P<0.01. (e) Id1 and Id3 mRNA levels in FACS-sorted splenic DC and MDSC populations, as determined by qPCR analysis, (n=6, one-way analysis of variance (ANOVA), *P<0.05, **P<0.01, ***P<0.001). (f) Id1 protein levels in lysates from naive and B16F10-bearing CD11b+ bead-sorted splenocytes as determined by western blot and densitometric analyses (unpaired t-test *P<0.01). (g) In vitro differentiation of Lin− haematopoietic progenitors isolated from C57BL/6 mice, cultured for 6 days in the presence of B16F10 melanoma TCM (25% v/v), and analysed for DC and MDSC content by flow cytometry (n=6, ANOVA, ***P<0.001). (h) Id1 mRNA relative expression levels of day 6 Lin− cells differentiated in the presence B16F10-conditioned media compared with control media, as determined by qPCR analysis (means±s.e.m., n=6, unpaired t-test, *P<0.05).