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. 2015 Apr 29;6:6840. doi: 10.1038/ncomms7840

Figure 3. Id1 overexpression induces a DC/MDSC imbalance.

Figure 3

Flow cytometry analysis of spleens from mice transplanted with Id1-overexpressing and control vector-transduced Lin− BM cells for (a) frequency and absolute numbers of DCs and (b) MDSCs (unpaired t-test, **P<0.01). (c) Representative percentages of MDSCs in GFP-positive splenocytes from mice transplanted with Id1-overexpressing Lin− cells and control vector splenocytes. (d) In vitro differentiation of Lin− haematopoietic progenitors from C57BL/6 mice transduced with lentiviral or control and Id1-overexpressing vectors overnight, cultured for 6 days and analysed for DC and MDSC content by flow cytometry (n=6, analysis of variance, **P<0.01). (e) Gene expression analysis of Lin− cells transduced with Id1-overexpressing and control vectors after 6 days of in vitro differentiation by qPCR analysis (means±s.e.m., n=6, unpaired t-test, **P<0.01, *P<0.05; NS, not significant). Four independent experiments were performed.