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. 2015 Mar 12;200(1):123–134. doi: 10.1534/genetics.115.176172

Figure 1.

Figure 1

Identification of the minimal trx-1 promoter region necessary for gene expression in ASJ neurons. (A) Bright-field (BF, top) and fluorescence (bottom) images of Ptrx-1(1kb)::gfp transgenic animals. ASJR (ASJ right) and ASJL (ASJ left) neurons are indicated. When expressed from the trx-1 promoter, GFP is found in the entire cell: in cilia, dendrites, cell bodies, and axons. Bar, 20 μm. (B) Identification of the minimal trx-1 promoter region that drives gene expression in ASJ neurons. Decreasing length fragments of the trx-1 promoter fused to the GFP-coding sequence have identified an ASJ minimal promoter (dotted blue line) located at position −200 to −84 upstream of the trx-1a ATG codon. (C) Serial deletions within the trx-1 minimal promoter region previously identified reveal the sequence −200 to −173 (dotted blue line) upstream of the trx-1a ATG codon as necessary for gene expression in ASJ neurons. For quantification, at least 50 animals per line were analyzed. (D) Transgenic animals expressing a 3× tandem construct of the sequence −200 to −173 (necessary for gene expression in ASJ) fused to GFP show fluorescence in head neurons with morphology and position consistent with being ASJ. The asterisk indicates the ASJ cell body, and the arrowhead points to the ASJ dendrite. Bar, 5 μm.