Figure 7.

Spontaneous suppressors of hst3∆ hst4∆ mutant phenotypes exhibit reduced Rad53 activity. (A) Fivefold serial dilutions were spotted on YPD plates containing the indicated concentrations of genotoxic agents and incubated at either 25 or 37°. TS is the starting hst3Δ hst4Δ mutant strain from which spontaneous thermoresistant (Tr) suppressors were isolated. (B) Immunoblotting was prepared from whole-cell lysates of exponentially growing cells. Ponceau S staining is shown as a loading control.