Table 5. Calculated relative abundance (percent of total histone H3) for H3 K79 methylation and H3 K56ac^a.

	Yeast genetic backgroundb
	hst3∆ hst4∆ H3/H4 WT	hst3∆ hst4∆ H3/H4K16R	hst3∆ hst4∆ H3K79R/H4
	(ASY2737)	(ASY2745)	(ASY2749)
H3K79Me0 ± SEMc,d	13.1 ± 0.4	10 ± 1	NA
H3K79Me1 ± SEMc,d	4.9 ± 0.1	8 ± 1	NA
H3K79Me2 ± SEMc,d	11.6 ± 0.4	26 ± 2	NA
H3K79Me3 ± SEMc,d	70.4 ± 0.1	56 ± 4	NA
H3K56Ac ± SEMc,e	80.3 ± 0.7	92.4 ± 0,5	88.3 ± 0.3

NA, not applicable.

^aThe abundance of each peptide was assessed by mass spectrometry of total histone H3 purified from each strain in buffers containing a cocktail of deacetylase inhibitors (see Materials and Methods).

^bStrains ASY2737, ASY2745, and ASY2749 are in the same genetic background (see Table 1).

^cStandard error of the mean of two mass spectrometry technical replicates.

^dThe values for the different forms of H3K79 reflect the relative abundance of a given isoform (e.g., H3K79me0) expressed as a percentage of the abundance of all H3K79 isoforms (K79me0+me1+me2+me3). For technical reasons, these values should not be equated stoichiometries. See Materials and Methods for a more detailed explanation.

^eThe values for H3K56Ac reflect stoichiometries, i.e., the fraction of all H3 molecules that are K56 acetylated. This is expressed as percentages obtained as follows: abundance of K56Ac divided by abundance of K56Ac + K56Pr. See Materials and Methods for a more detailed explanation.