The organization of the Gimap3 gene in mice. (A) A graphical illustration of the Gimap3 gene, mRNA, and protein derived from BALB/c and CAST/Ei alleles. Red indicates the main coding sequence for Gimap3, while purple indicates the sequence of the uORF. CC, coiled coil; TM, transmembrane; AIG, Gimap family domain. (B) Alignment of the predicted starter methionine from Gimap3 and Gimap5 across selected mammalian taxa against the Kozak consensus sequence. (C) A graphical illustration of the Gimap3 and Gimap5 proteins with color differences indicating sequence divergence between the two proteins. (D) An amino acid alignment of the C terminus of Gimap3 against Gimap5 from selected mammalian taxa. Predicted transmembrane domain with flanking charged amino acids: positive in red and negative in blue. Amino acids in Gimap3 that are conserved with Gimap5 appear in white type on a black background.