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. 2015 Mar 25;200(1):221–235. doi: 10.1534/genetics.115.175596

Figure 3.

Figure 3

Gimap3 expression affects mtDNA segregation in mouse spleen. (A) The expression level of the BALB/c Gimap3 mRNA relative to CAST/Ei Gimap3 by semiquantitative RT–PCR in F2 (BALB/c × CAST/Ei) mice heterozygous at the Gimap3 locus. Mice were either positive (+) (n = 51) or negative (−) (n = 19) for mtDNA segregation. Mann–Whitney Test, P < 0.001. (B) Immunoblotting of total cell lysates from the spleen of BALB/c, CAST/Ei or F2 (BALB/c × CAST/Ei) mice. A NIH3T3 cell line was transduced with retroviruses expressing HA-tagged version of both the BALB/c Gimap3 and CAST/Ei Gimap3 was used as positive control. (*) Extended exposure with the CAST Gimap3 antibody. (C) Northern blot of total spleen RNA from CAST/Ei and BALB/c mice with three independent Gimap3 probes (a–c) and a control probe for Atp5B. As positive and negative controls for the Gimap3 probes total RNA was loaded from MEFs ectopically expressing the coding sequence (CDS) of BALB/c Gimap3 and Gimap5. On the right, a graphical illustration of the Gimap3 gene and location of the oligonucleotide probes.