Figure 6.

Genetic loss of Gimap5 affects Gimap3 and mtDNA segregation. (A) Northern blot of spleen total RNA from wild-type Gimap5, Gimap5^tm1Wlr/WT, and homozygote Gimap5^tm1Wlr mice hybridized with Gimap3 (b) probe and control probe Atp5B. Right: Quantification of Gimap3 mRNA compared to Atp5B. (B) Decreased selection against the NZB mtDNA genome in the bone marrow and spleen of Gimap5^tm1Wlr/WT compared to wild-type littermate controls. n = 18 for each group; (*) P < 0.01 two-tailed t-test; means are indicated by the line through the scatter plot. Mice were 70 days of postnatal age at the time of sampling. (C) A representative immunoblotting of Gimap3 from whole-cell lysates from isolated splenocytes of wild type, Gimap5^tm1Wlr/WT and homozygote Gimap5^tm1Wlr mice. Left: Quantification of Gimap3 abundance compared to Calnexin.