Figure 8.

Imaging of live C. glabrata strains carrying various Sir3-GFP alleles. Eight strains carrying different versions of Sir3-GFP, as well as an untagged Sir3 control strain, were grown to stationary phase in liquid culture. Live cells were imaged using differential interference contrast bright-field and fluorescence microscopy. GFP fluorescence is shown with both the contrast settings as captured (automated contrast) and adjusted so each strain has the same contrast levels (adjusted contrast). The maximum and minimum contrast settings for each strain are listed on the automated contrast panel. The adjusted contrast panels all have a minimum of 202 and a maximum of 727. Bar, 5 μm.