Figure 4.

Number of proteins differentially expressed among diploid and allopolyploid species. Bold text indicates the total number and percentage of proteins differentially expressed in each comparison. The total number of proteins is partitioned into both directions using bold text; e.g., of the 153 proteins differentially expressed between G. hirsutum and G. arboreum in A, 69 and 84 were up-regulated in the former and latter species, respectively. For 2-DE comparisons at 10 (A) and 20 dpa (B), percentages were calculated based on a total of 1323 spots detected on gels. For iTRAQ comparisons at 10 (C) and 20 dpa (D), percentages were calculated based on 1652 nonredundant proteins. Using coelectrophoresis as control, additivity of protein expression in allopolyploids was tested at 10 (E) and 20 dpa (F) for 2-DE data.