Figure 3. dilp6 Mutant Phenotypes.
(A) Schematic representation of the dilp6 locus and molecular nature of the mutations. The gene structure of dilp6 is shown, with protein coding regions represented by open boxes and untranslated regions by filled boxes. An arrow indicates the orientation of the gene. Three 3′ flanking genes are depicted in gray boxes, along with a short putative exon of a 5′ flanking gene, phl, marked with an asterisk. The site of P element insertion (KG04972) is marked with an inverted triangle. A part of the P element is still present in dilp63932 (open triangle).
(B) Relative dilp6 expression levels in the mutants at 0 hr APF, as assessed by qRT-PCR. N.D., not detected.
(C) Hemolymph sugar (glucose + trehalose) concentrations of control, dilp63932, and dilp64591 male wandering larvae 36 hr after L3 ecdysis. Hemolymph was collected from batches of 15 larvae.
(D) Body weight of control, dilp63932, and dilp64591 male flies. Flies were weighed in batches of 10–30, and the average weight per fly was calculated.
(E–G) Wing area (E), cell size (F), and cell number (G) of control, dilp63932, and dilp64591 male flies.
(H and I) Developmental changes in the wet weight (H) and dry weight (I) of control and dilp63932 animals. Animals were weighed in batches of 10–50, and the average weight per animal was calculated.
(J) Developmental changes in the percentage of difference in dry weight between control and dilp63932 animals, calculated from (I).
All values are the means and SD (n = 3 batches [B], 4 batches [C, D, H, I], or 20 wings [E–G]; Student’s t test; *p < 0.05, **p < 0.01).