Figure 1.

Cell adhesion status during KV organogenesis. KV organogenesis is divided into four steps. First, 20–30 DFCs form a cluster through the formation of adherens junction. A subset of DFCs binds to EVL through the formation of tight junction. Second, the cluster becomes compact. Multiple focal points that enrich tight junction components are generated between some DFCs. Third, these points are then rearranged into a single focal point. Fourth, an internal lumen is generated. During collective DFC migration, adherens junction, tight junction, and cell-ECM interaction are generated in a migrating DFC clusters.