Table 4.
Tissue AGAT and GAMT Expression
| Brain | Heart | Gastrocnemius muscle | |||||||
|---|---|---|---|---|---|---|---|---|---|
| Non-pregnant | Pregnant | Stats | Non-pregnant | Pregnant | Stats | Non-pregnant | Pregnant | Stats | |
| AGAT mRNA | 1.00 ± 0.06 | 0.48 ± 0.10 | <0.01 | 1.00 ± 0.25 | 0.20 ± 0.02 | <0.01 | 1.00 ± 0.11 | 0.40 ± 0.03 | <0.01 |
| AGAT Protein | 1.00 ± 0.12 | 0.73 ± 0.18 | NS | 1.00 ± 0.26 | 0.46 ± 0.07 | 0.054 | 1.00 ± 0.45 | 1.23 ± 0.65 | NS |
| GAMT mRNA | 1.00 ± 0.15 | 0.23 ± 0.05 | <0.001 | 1.00 ± 0.29 | 0.68 ± 0.05 | NS | 1.00 ± 0.13 | 0.78 ± 0.10 | NS |
| GAMT Protein | 1.00 ± 0.46 | 0.20 ± 0.07 | 0.05 | 1.00 ± 0.37 | 2.27 ± 0.35 | <0.05 | 1.00 ± 0.42 | 0.77 ± 0.28 | NS |
mRNA and protein expression of AGAT and GAMT in the brain, heart and skeletal muscle of non-pregnant and pregnant spiny mice at term. Data are expressed relative to the house keeping gene/protein. Values are means ± SEM; n = 6/group. Statistical analysis Students t-Test. Significance P ≤ 0.05.