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Representative flow cytometry gating strategy for identification of B cell (CD19+) subsets: naïve B cells (CD10−CD27−CD21+CD20+), tissue-like memory B cells (CD10−CD27−CD21−CD20+), activated memory B cells (CD10−CD27+CD21−CD20+), resting memory B cells (CD10−CD27+CD21+CD20+), and plasma cells (CD10−CD27+CD21−CD20−) in peripheral blood mononuclear cells.
