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. 2015 Jan 5;66(10):2957–2966. doi: 10.1093/jxb/eru512

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 3. — The deficiency of NTRC and Srx has opposite effects on chloroplast 2-Cys Prxs overoxidation. (A) Arabidopsis wild-type, ntrc, srx, and ntrc-srx double mutant plants were grown for 10 days under long-day conditions in plates. Protein extracts (12.5 µg) from seedlings were subjected to SDS-PAGE (12% acrylamide), supplemented with 4M urea under non-reducing conditions, electrotransferred onto nitrocellulose filters, and probed with the anti-2-Cys Prx antibody. (B) The amount of overoxidized 2-Cys Prxs was determined on protein extracts (60 µg) from seedlings of the different lines, which were subjected to SDS-PAGE (12% acrylamide), supplemented with 4M urea under reducing conditions. Gels were electrotransferred onto nitrocellulose filters, and probed with the anti-SO_2/3 antibody. For each genotype, band intensity was quantified with the Scion Image analysis software (Scion Corporation) and normalized against that of the indicated band (arrow) in the ponceau S-stained membrane, which is shown as a loading control. Relative densities are referred to the intensity of the wild type, which was set at 1.