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. 2015 Jan 5;66(10):2957–2966. doi: 10.1093/jxb/eru512

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© The Author 2015. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — 2-Cys Prxs A and B from Arabidopsis show similar sensitivity to overoxidation. Purified recombinant 2-Cys Prxs A and B (0.125 μg of protein) from Arabidopsis were incubated for 30min with or without 20mM DTT, then samples were incubated in the presence of the indicated concentrations of hydrogen peroxide for 30min. Samples were then subjected to SDS-PAGE (15% acrylamide) under non-reducing conditions, electrotransferred on to nitrocellulose filters and probed with anti-2-Cys Prx or anti-SO_2/3 antibodies, as indicated. dim, dimer; mon, monomer.