Fig. 7.

Effect of light on the redox status of chloroplast 2-Cys Prxs from Arabidopsis ntrc mutant plants. Arabidopsis ntrc mutant seedlings that were grown under standard long-day conditions for 10 days were incubated during two consecutive days, the second one with continuous light (A) or continuous darkness (B) and samples were harvested at 4-h intervals, as indicated. Protein extracts (40 µg of protein) were subjected to SDS-PAGE (12% acrylamide) supplemented with 4M urea under non-reducing conditions, electrotransferred onto nitrocellulose filters, and probed with the anti-2-Cys Prx antibody. dim, dimer; mon, monomer.