Table 2.
Identification of LjGpxs that interact with LjTrxh4
Proteins from nodule extracts that interact with LjTrxh4 were trypsinized and the resulting peptides were analysed by LC-MS/MS using the DDAM (data-dependent acquisition mode) or the TM (target mode). The mass to charge ratio (m/z) of the fragmented peptide ions are indicated. Three independent experiments were conducted, each one corresponding to a different nodule sample. +, ++, +++, positive identifications in one, two, or three experiments.
| Proteins and peptides | m/z | DDAM | TM |
|---|---|---|---|
| LjGpx1 | |||
| FKAEFPVFDKVDVNGDSAAPLYK | 853.10 | ++ | +++ |
| AEFPVFDKVDVNGDSAAPLYK | 761.38 | ++ | +++ |
| VDVNGDSAAPLYK | 674.84 | ++ | +++ |
| GNDVNLGDYK | 547.76 | +++ | +++ |
| FLVDKEGNVVER | 702.88 | ++ | +++ |
| LjGpx2 | |||
| FKSEFPIFDKIEVNGENSAPLYK | 891.46 | + | +++ |
| SEFPIFDKIEVNGENSAPLYK | 799.73 | + | |
| IEVNGENSAPLYK | 717.37 | ++ | |
| GSDVDLSTYK | 1084.52 | ++ | +++ |
| WGIFGDDIQWNFAK | 848.90 | +++ | +++ |
| FLVDKDGQVVDR | 695.87 | ++ | +++ |
| LjGpx3 | |||
| SLYDFTVK | 486.75 | + | +++ |
| ELNILYEK | 511.28 | + | +++ |