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. 2015 May 7;5:10149. doi: 10.1038/srep10149

Figure 5. Correlation functions from 2c2f lsFCS experiments on HEK293T cells to study binding of three ligand-receptors pairs.

Figure 5

(a) hDkk2-GFP binding to LRP6-mCherry, (b) zfDkk1-GFP to LRP6-mCherry, (c) zfDkk1-GFP to ROR2-mCherry. For each column, the data shown are, from top to bottom: autocorrelation and dual-focus cross-correlation functions (red channel, receptor), autocorrelation and dual-focus cross-correlation functions (green channel, ligand), and dual-color and dual-color dual-focus cross-correlation functions (plotted in blue). Error bars indicate standard deviations from multiple data sets. Symbols: experimental data, lines: fitted correlation functions.