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. 2015 Apr 23;16(Suppl 2):S2. doi: 10.1186/1471-2156-16-S2-S2

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Copyright © 2015 Gardner et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Signaling activity of various PhoU mutants. Triplicate cultures were grown in LB and assayed for Bacterial Alkaline Phosphatase activity. BW25113 is a wildtype control. Other strains were STACΔphoU with p116U2 (wildtype phoU), pKG116 (empty parent plasmid), and various p116U2 plasmids with specific mutations in phoU (A147E, A147K, R148A, R148E, and D150A). Error bars represent standard deviations. Values significantly greater than p116U2 are labeled with * = p-value of < 0.001 compared to p116U2 with a two-tailed T-test.