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. 2015 Apr 23;16(Suppl 2):S2. doi: 10.1186/1471-2156-16-S2-S2

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Copyright © 2015 Gardner et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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PAS domain scanning mutagenesis. A. Predicted secondary structure of the PhoR PAS domain with alpha helixes labeled with yellow arrows and β-sheets labeled with red cylinders. B. Scanning mutagenesis of the PhoR PAS domain used BACTH to identify regions of the protein that are essential for interaction with PhoU. Every two amino acids were changed to code for alanine and cysteine. Each construct was tested in triplicate for interaction with PhoU. Blue bars represent samples that had less than 40% of the activity of unmutated PhoR.