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Active site of wild-type Adh1p from S. cerevisiae . Residues mutated during this work (Val59, Ser110, Leu117, Gln148, Ile152 and Tyr295) are presented as stick models (orange) together with NAD(H) analogue nicotinamide-8-iodo-adenine-dinucleotide and substrate analogue trifluoroethanol (white). Structural and active-site zink are shown as spheres (green). The image was created in PyMol (www.pymol.org) from the atomic coordinates in 2HCY.
