Table 4.
Kinetic parameters of the Adh1p-variants with acetaldehyde, HMF and furfural as substrates
| Variant | Substrates | Vmax (mU/mg) | Km (mM) | Ki (mM) | [S] Vmax , (obsV max )* | RSQ |
|---|---|---|---|---|---|---|
| natAdh1p | Acetaldehyde | 45070 | 0.22 | 12.53 | 1 (35300) | 0.987 |
| HMF | Not detected | |||||
| Furfural | 6530 | 18.8 | 1363 | 30 (4345) | 0.970 | |
| mutAdh1p | Acetaldehyde | 4736 | 1.69 | 49 | 10 (3700) | 0.968 |
| HMF | 3120 | 4.30 | 9.82 | 5–10 (1320) | 0.984 | |
| Furfural | 1625 | 0.04 | 1.07 | 0.25 (1230) | 0.974 | |
| mutAdh1p-rev117 | Acetaldehyde | 23060 | 1.74 | 96 | 10–25 (17600) | 0.979 |
| HMF | 6320** | 4.28 | n.d | 10 (4900) | 0.975 | |
| Furfural | 11700 | 0.33 | 4.40 | 1 (7600) | 0.992 | |
| natAdh1p-m110, 295 | Acetaldehyde | 16509 | 0.83 | 46 | 5 (14300) | 0.970 |
| HMF | 11090 | 9.45 | 20.80 | 10–20 (4560) | 0.999 | |
| Furfural | 5657 | 0.13 | 1.32 | 0.25–0.5 (3480) | 0.984 | |
| mutAdh1p-rev110,117 | Acetaldehyde | 32455 | 3.03 | 56 | 10 (22000) | 0.974 |
| HMF | 3640 | 13.10 | 27.30 | 20 (1550) | 0.980 | |
| Furfural | 7792 | 0.34 | 6.66 | 1 (5320) | 0.986 | |
*[S]Vmax – substrate concentration (mM) at observed Vmax (obsVmax, mU/mg), **Vmax and Km values derived from a model without substrate inhibition factor, see text for details.