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. 2014 Aug 9;13:112. doi: 10.1186/s12934-014-0112-5

Table 7.

Plasmids used in the study

Plasmid Construction background Reference
YEplacHXT (reference plasmid) YEplac195, HXT7p-PGKt URA3 [31]
YEplacHXT-natADH1 YEplacHXT, native ADH1 This study
YEplacHXT-mutADH1 YEplacHXT, mutated ADH1 [10]
YEplacHXT-mutADH1-rev110 YEplacHXT, mutated ADH1, P110S This study
YEplacHXT-mutADH1-rev117 YEplacHXT, mutated ADH1, S117L This study
YEplacHXT-mutADH1-rev295 YEplacHXT, mutated ADH1, C295Y This study
YEplacHXT-natADH1-m110 YEplacHXT, native ADH1, S110P This study
YEplacHXT-natADH1-m295 YEplacHXT, native ADH1, Y295C This study
YEplacHXT-natADH1-m110,295 YEplacHXT, native ADH1, Y295C, S110P This study
YEplacHXT-mutADH1-rev110, 117 YEplacHXT, mutated ADH1, P110S, S117L This study