FIGURE 11.

Effect of cystathionine on cell viability and intracellular glutathione levels in MEF derived from WT and xCT-deficient (KO) mice. WT and KO MEF were cultured for 24 h in the routine culture medium and then cultured in the cystine-free medium with (●) or without (○) 0.1 mm cystathionine for the indicated time periods in the absence of 2-mercaptoethanol. A and B, cell viability of WT MEF and KO MEF, respectively; C and D, intracellular glutathione levels of WT and KO MEF, respectively. Each point represents the mean ± S.D. (error bars) (n = 4–8). *, p < 0.05; **, p < 0.01 compared with control (without cystathionine).