FIGURE 2.

Expression of Cbs, Cgl, and xCT mRNA in the thymus, spleen, and liver of WT and xCT-deficient (KO) mice. Thymus, spleen, and liver were collected from male xCT WT and KO mice. Total RNA was extracted, and Cbs, Cgl, and xCT mRNA expression was determined by quantitative RT-PCR as described under “Experimental Procedures.” GAPDH was used as the internal standard. Cbs and Cgl mRNA expression is represented as -fold of that of liver of wild type, and xCT mRNA expression is represented as -fold of that of thymus of wild-type (n = 3). Error bars, S.D.