FIGURE 10.

C-term phosphomimetic mutant trafficking phenotypes. GFP-WTATP7B (from three independent experiments; also shown in Fig. 5) and the two C-terminal mutants (from two independent experiments performed in parallel) shown in Fig. 9 were expressed and evaluated for copper-directed trafficking. Wide field images of 50–100 cells for each treatment were evaluated, and graphs illustrate the percentage of cells with a medium/strong presence in TGN (A), vesicles (B), or apical region (C) under the three copper conditions. Totals exceed 100% because some cells showed a mixed phenotype. The 8-mimetic shows somewhat more vesicles in the +copper (+Cu) condition than either the 3-mimetic or WT.