FIGURE 11.

Introduction of 13 inactivating residues (Ala or Gly) in the C terminus does not disrupt copper-responsive trafficking. GFP-ATP7B mutants with three (left column) or 13 (middle column) Ala/Gly inactivating mutations were expressed in WIF-B cells, treated under various copper conditions, and then stained as in Fig. 3. Single confocal planes are shown. n, nucleus; arrows, apical surface; APN, aminopeptidase N. The right column shows the 13 S/T>A/G mutant with two additional sites inactivated (S1116A/S1121A present in the N domain).