FIGURE 5.

Deletion/mutation of N-terminal metal binding domains does not disrupt copper-responsive appearance at the apical domain. A–F′, WIF-B cells expressing the GFP-ATP7B constructs shown in A were fixed, stained, and imaged as in Fig. 3. Images on the left (B–E) show the GFP channel (green); images on the right (B′–E′) show overlays with the TGN (red) and apical (blue) markers. Cells were incubated overnight in 10 μm BCS (−Cu; top row) or BCS followed by 10–20 μm CuCl₂ for 1–1.5 h (+Cu; middle row); some were then washed and treated with 10 μm BCS to initiate retrograde trafficking (+Cu → −Cu; bottom row). n, nucleus; arrows, apical surface; APN, aminopeptidase N.