FIGURE 6.

N-term metal binding domain mutant trafficking phenotypes. GFP-WTATP7B (from three independent experiments) and the two N-terminal mutants (from two independent experiments performed in parallel) shown in Fig. 5 were expressed and evaluated for copper-directed trafficking. Wide field images of 50–100 cells for each treatment were evaluated, and graphs illustrate the percentage of cells with a medium/strong presence in TGN (A), vesicles (B), or apical region (C) under the three copper conditions. Totals exceed 100% because some cells showed a mixed phenotype. Arrows indicate where and in which copper condition the mutants differ from WT. The 1–63 Δ1–4MBD C>S mutant has a smaller fraction of cells with a strong/medium level at the apical region (*).