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Introduction of eight negative charges at putative C-term phosphorylation sites does not disrupt copper-directed trafficking. GFP-ATP7B mutants with three or eight negative charges (3- and 8-mimetic) were expressed in WIF-B cells, treated under various copper conditions, and then stained as in Fig. 3. Single confocal planes are shown. n, nucleus; arrows, apical surface; APN, aminopeptidase N.
