TABLE 1.

The two intrinsic activities of DnaK

The two intrinsic activities of DnaK are as follows: ATPase and peptide substrate binding activities.

	ATPase activity kcat	Peptide binding Kd
	×10−2/min	μm
WT	1.04 ± 0.076	1.37 ± 0.04
R56A	3.61 ± 0.260	1.25 ± 0.06
T301A	0.89 ± 0.089	1.28 ± 0.06
N537A	0.91 ± 0.094	0.72 ± 0.07
D540A	0.99 ± 0.109	0.75 ± 0.06