FIGURE 8.

Single molecule force spectroscopy of purified P1 proteins. A, schematic of the surface chemistry used to functionalize model substrates with P1 and AFM tips with anti-P1 targeting the apical head (mAb 1-6F). V, variable. B, adhesion force histograms (n = 1024) and retraction force curves (inset) recorded between the P1 substrate and the anti-P1 tip. C, scatter plot of the specific adhesion forces as a function of the rupture length (21% of a total of n = 1024 force curves). Similar results were obtained in several independent experiments using different tip and sample preparations.