A–D, flash response families of dark-adapted control (A), GCAPs−/− (B), Rec−/− (C), and GCAPs−/−Rec−/− (D) M-cones from transretinal recordings. Red traces in each panel correspond to responses evoked by identical intensity of 500-nm light. E and F, fractional single photon response (E) and normalized responses (F) of control (black), Rec−/− (red), GCAPs−/− (green), and GCAPs−/−Rec−/− (blue) M-cones. For comparison, the responses of GCAP-deficient cones (B and D) are reproduced from Ref. 23, and C is reproduced from Fig. 2B.