FIGURE 1.

A, RT-PCR across exons 30–35 could produce a fragment of 382 bp when exon 33 (33 bp) is present. Exons 31 and 32 are mutually spliced exons. Digestion at exon 32 with NsiI will generate two fragments of 68 and 314 bp. B, partial digestion with NsiI was observed in both NH and AH. Exon 32 is expressed higher in AH as more PCR produce was digested. Equal amounts of DNA from both AH and NH were digested under same conditions in parallel.