FIGURE 9.
Cav1.233L channels down-regulates the reference Cav1.2 channels by enhancing protein degradation via ubiquitin-proteasome system. A, Western blot for the expression of the reference Cav1.2 channels with or without Cav1.233L channels and proteasome inhibitor MG132 (3 μm). B, Western blot for the expression of Cav1.3 channels with or without Cav1.233L channels and MG132. C, immunoprecipitation (IP) using anti-Cav1.2 antibody to detect ubiquitinated (Ub) Cav1.2 channels with or without Cav1.233L channels and MG132 treatments. Ubiquitinated Cav1.2 channels were labeled by immunoblotting (IB) using a mouse anti-ubiquitin antibody. D, ubiquitination of Cav1.3 channels with or without Cav1.233L channels and MG132 treatments. The results are representative of three independent experiments.