FIGURE 2.

TAZ1 and YME1 double mutant cells are synthetically sick. A, tetrad separation of meiotic spores from a cross between taz1Δ and yme1Δ yeast shows slow growing taz1Δ yme1Δ mutants (indicated by white circles) in fermentable YPD medium. Each column has four colonies arising from a single tetrad (ascus) that resulted from the cross between taz1Δ and yme1Δ cells. The single mutants taz1Δ and yme1Δ carry nourseothricin and kanamycin resistance markers, respectively. Growth resistance to the addition of both the antibiotics G418 and nourseothricin to YPD medium confirmed the genetic make up of the double mutant colonies. B, spot assay shows that taz1Δ yme1Δ double mutants are synthetically sick in YPD medium. The indicated strains were grown to early stationary phase, 0.9–1.0 A₆₀₀, and then resuspended in SC medium at 0.45 A₆₀₀. This dilution was then serially diluted (1:10) and spotted on YPD plates and grown for 3–5 days at 25 °C. C, the indicated strains were grown to early stationary phase, 0.9–1.0 A₆₀₀, and then suspended in minimal medium at 0.45 A₆₀₀. This dilution was then serially diluted (1:10) and spotted on SC medium with 2% lactate and grown for 3–5 days at 25 and 37 °C.