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. Author manuscript; available in PMC: 2016 Apr 16.
Published in final edited form as: Mol Cell. 2015 Apr 9;58(2):255–268. doi: 10.1016/j.molcel.2015.03.011

Figure 6. Targeting of PTEN to PI(3)P Is Necessary for Function in Cells.

Figure 6

(A) Overexpression of multiple GFP-PTEN fusion proteins and their sub-cellular localization. Z = 1. Scale bar, 10 μm.

(B) Overexpression of PTEN constructs followed by FACS and interrogation of AKT phosphorylation. Fusion of mCBR3 PTEN to FYVE domains restores the ability of PTEN to efficiently antagonize PI3-K/AKT-mediated signaling.

(C) EGF-Cy5 internalization leads to colocalization with GFP-2×FYVEHRS after 15 min (arrows). Z = 1. Scale bar, 10 μm.

(D) Colocalization of ChFP-C2PTEN domain with EGF-Cy5 (arrowheads) and of ChFP-C2PTEN domain, EGF-Cy5, and GFP-2×FYVEHRS (arrows) is seen at 15 min post-EGF-Cy5 internalization. Z = 1. Scale bar, 10 μm.

See also Figure S4.