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. 2015 May 7;10(5):e0119396. doi: 10.1371/journal.pone.0119396

Fig 5. The potentiation of the Akt/mTOR pathway at E90 and D100.

Fig 5

A. To detect the activation of the PI3K/Akt/mTOR signaling pathway, E90 and D100 longissimus dorsi were prepared and resolved by 10% SDS-PAGE. The PVDF membrane was probed with antibodies to phosphor-Akt and phosphor-mTOR, respectively. Antibodies against Akt, mTOR and β-actin were used to verify the equal protein loading. B. All quantitative results were normalized byβ-actin and were shown as mean ± SD from three independent experiments.